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當(dāng)前位置： Millipore >>> Millipore/GF094 | Vascular Endothelial Growth Factor, Recombinant human, 165aa isoform/GF094/10 µg

Millipore/GF094 | Vascular Endothelial Growth Factor, Recombinant human, 165aa isoform/GF094/10 µg

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貨號(hào): GF094

品牌: Millipore

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Description
CatalogueNumber	GF094
Replaces	GF025
BrandFamily	Chemicon®
TradeName	Chemicon
Description	VascularEndothelialGrowthFactor,Recombinanthuman,165aaisoform
Overview	VascularEndothelialCellGrowthFactor(VEGF)isasecretedhomodimericproteinsecretedbyavarietyofvascularizedtissues.ThereportedactivitiesofVEGFincludestimulationofendothelialcellgrowth,angiogenesis,andcapillarypermeABIlity.HumanVEGFisa38.2kDahomodimericproteinconsistingoftwo165aminoacidpolypeptidechains.
AlternateNames	VEGF

ProductInformation
Presentation	Lyophilizedfromsterilefiltered20mMaceticacid,containingnoadditives.

StorageandShippingInformation
StorageConditions	Thelyophilizedpowderisstableforafewweeksatroomtemperature,butbeststoredat-20°Cforuptooneyearfromdateofreceipt.Afteraquickspin,reconstitutewithsterilewatertoaconcentrationof0.1-1.0mg/mL.Reconstitutedmaterialcanbestoredat2°C-8°Cforoneweek,butundilutedaliquotsat-20°Carerecommended.Avoidrepeatedfreeze/thawcycles.

Applications
Application	VascularEndothelialCellGrowthFactor(VEGF)isasecretedhomodimericproteinsecretedbyavarietyofvascularizedtissues.
KeyApplications	CellCulture
ApplicationNotes	Formostinvitroapplications,humanVEGFexertsitsBIOLOGicalactivityintheconcentrationrangeof0.1to50.0ng/mL.Respondingcellsare(partiallist):Endothelialcells.

BiologicalInformation
Purity	Greaterthan98%bySDS-PAGE.Endotoxinlevelislessthan0.1ngperμg(1EU/ug)ofVEGF
Source	E.coli
SpecificActivity	ThebiologicalactivityofMillipore'shumanVEGFwasdeterminedbyitsmitogenicactivityonhumanumbilicalveinendothelialcells(HUVEC).Mitogenicactivitywasobtainedinaconcentrationrangeof1.0-8.0ng/mL.
EntrezGeneNumber	NM_001025366.1 NM_001025367.1 NM_001025368.1 NM_001025369.1 NM_001025370.1 NM_001033756.1 NM_003376.4
EntrezGeneSummary	ThisgeneisamemberofthePDGF/VEGFgrowthfactorfamilyandencodesaproteinthatisoftenfoundasadisulfidelinkedhomodimer.Thisproteinisaglycosylatedmitogenthatspecificallyactsonendothelialcellsandhasvariouseffects,includingmediatingincreasedvascularpermeability,inducingangiogenesis,vasculogenesisandendothelialcellgrowth,promotingcellmigration,andinhibitingapoptosis.ElevatedlevelsofthisproteinislinkedtoPOEMSsyndrome,alsoknownasCrow-Fukasesyndrome.Mutationsinthisgenehavebeenassociatedwithproliferativeandnonproliferativediabeticretinopathy.Alternativelysplicedtranscriptvariants,encodingeitherfreelysecretedorcell-associatedisoforms,havebeencharacterized.Thereisalsoevidencefortheuseofnon-AUG(CUG)translationinitiationsitesupstreamof,andin-framewiththefirstAUG,leADIngtoadditionalisoforms.
GeneSymbol	VEGFA MGC70609 VPF VEGF VEGF-A
UniProtNumber	P15692
UniProtSummary	FUNCTION:SwissProt:P15692#Growthfactoractiveinangiogenesis,vasculogenesisandendothelialcellgrowth.Inducesendothelialcellproliferation,promotescellmigration,inhibitsapoptosis,andinducespermeabilizationofbloodvessels.BindstotheVEGFR1/Flt-1andVEGFR2/Kdrreceptors,heparansulfateandheparin.Neuropilin-1bindsisoformsVEGF-165andVEGF-145. SIZE:232aminoacids;27042Da SUBUNIT:Homodimer;disulfide-linked.AlsofoundasheterodimerwithPlGF(Bysimilarity). SUBCELLULARLOCATION:Secreted.Note=VEGF121isacidicandfreelysecreted.VEGF165ismorebasic,hasheparin-bindingpropertiesand,althoughasignicantproportionremainscell-associated,mostisfreelysecreted.VEGF189isverybasic,itiscell-associatedaftersecretionandisboundavidlybyheparinandtheextracellularmatrix,althoughitmaybereleasedasasolubleformbyheparin,heparinaseorplasmin. TISSUESPECIFICITY:TheVEGF189,VEGF-165andVEGF-121isoformsarewidelyexpressed,whereastheVEGF206andVEGF-145areuncommon. SIMILARITY:SwissProt:P15692##BelongstothePDGF/VEGFgrowthfactorfamily.
StemCellType	NeuralStemCells MesenchymalStemCells HematopoieticStemCells
MolecularWeight	38.2kDa

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