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Millipore/FC010 | Human Plasma Fibronectin Purified Protein/FC010/1 mg
  • Millipore/FC010 | Human Plasma Fibronectin Purified Protein/FC010/1 mg

Millipore/FC010 | Human Plasma Fibronectin Purified Protein/FC010/1 mg

價(jià)格: ￥1260.00 市場(chǎng)價(jià): 2100.00
貨號(hào): FC010
品牌: Millipore
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    • Description
      CatalogueNumberFC010
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionHumanPlasmaFibronectinPurifiedProtein
      OverviewHumanfibronectin(HFN)issuitableforuseasanattachmentfactorinthepropagationofcellsinvitrowhenusedtocoatcellculturesurfaces,includingplasticware,glassware,andmicrocarrierbeads.
      ProductInformation
      PresentationLiquidin150mMNaCl,10mMsodiumphosphate,pH7.5,sterilefiltered,containingnopreservatives.
      StorageandShippingInformation
      StorageConditionsMaintainat2-8°Cforupto6monthsfromdateofreceipt.Donotfreeze.
      Applications
      ApplicationThisHumanplasmafibronectinisapurifiedprotein,usedasanattachmentfactorsuitableforcellpropagationinvitro.
      KeyApplications
      • CellCulture
      ApplicationNotesImmunochemicalstandard

      Cellattachmentandproliferationassaysonhumanendothelialcells,humankeratinocytesandhumandermalfibroblasts

      SuggestedProcedureforCoatingCellCultureware

      1.DeterminetheamountofHFNneededtocoatculturevesselsbymultiplyingthetotalsurfacearea(cm2)bythedesiredconcentration(μg/mL)ofHFN.Recommendedamountis2-10μg/cm2.

      2.Wetthesurfaceofeachculturevesseltobecoatedwithaminimumamountofsterilebalancedsaltsolution(serumandproteinfree)requiredtocovertheentirearea.

      3.IntroducetheproperCO2atmosphere,ifrequired.

      4.AddthecalculatedamountofHFNtoeachculturevessel.

      5.AllowHFNtoadsorbtothesurfaceofthevesselfor5-20minutes.

      6.Removeresidualbalancedsaltsolutionbeforeproceedingwithstandardcellcultureprocedures
      BIOLOGicalInformation
      Concentration1mg/mL
      PurityApproximately95%,asdeterminedbySDS-PAGE.Adoublebandof220kDaispresentunderreducedconditions.hFNispurifiedbyaffinitychromatographyongelatinagarose,followedbychromatographyonheparin-agarose.Plasmafromdonorshasbeenscreened,andshowntobenegativeforHIV,HTLV,HepatitisBandC.
      SourceHumandonorplasma.
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodesfibronectin,aglycoproteinpresentinasolubledimericforminplasma,andinadimericormultimericformatthecellsurfaceandinextracellularmatrix.Fibronectinisinvolvedincelladhesionandmigrationprocessesincludingembryogenesis,woundhealing,bloodcoagulation,hostdefense,andmetastasis.Thegenehasthreeregionssubjecttoalternativesplicing,withthepotentialtoproduce20differenttranscriptvariants.However,thefull-lengthnatureofsomevariantshasnotbeendetermined.
      GeneSymbol
      • FN1
      • DKFZp686O13149
      • DKFZp686H0342
      • DKFZp686I1370
      • MSF
      • FN
      • CIG
      • DKFZp686F10164
      • LETS
      • FINC
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P02751#Fibronectinsbindcellsurfacesandvariouscompoundsincludingcollagen,fibrin,heparin,DNA,andactin.Fibronectinsareinvolvedincelladhesion,cellmotility,opsonization,woundhealing,andmaintenanceofcellshape.InteractionwithTNRmediatesinhibitionofcelladhesionandneuriteoutgrowth(Bysimilarity).
      SIZE:2386aminoacids;262607Da
      SUBUNIT:Mostlyheterodimersormultimersofalternativelysplicedvariants,connectedby2disulfidebondsnearthecarboxylends;toalesserextenthomodimers.InteractswithFBLN1,AMBP,TNR,LGALS3BPandCOL13A1.
      SUBCELLULARLOCATION:Secreted,extracellularspace,extracellularmatrix.
      TISSUESPECIFICITY:PlasmaFN(solubledimericform)issecretedbyhepatocytes.CellularFN(dimericorcross-linkedmultimericforms),madebyfibroblasts,epithelialandothercelltypes,isdepositedasfibrilsintheextracellularmatrix.Ugl-Y1,Ugl-Y2andUgl-Y3arefoundinurine.DEVELOPMENTALSTAGE:Ugl-Y1,Ugl-Y2andUgl-Y3arepresentintheurinefrom0to17yearsofage.
      PTM:Sulfated.
      SIMILARITY:SwissProt:P02751##Contains12fibronectintype-Idomains.&Contains2fibronectintype-IIdomains.&Contains16fibronectintype-IIIdomains.
      StemCellType
      • HumanEmbryonicStemCells
      • MesenchymalStemCells
      • NeuralStemCells
      • HematopoieticStemCells
      • EpithelialCells
      • PancreaticStemCells
      • InducedPluripotentStemCells
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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