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當(dāng)前位置： Millipore >>> Millipore/ECM580 | QCM Haptotaxis Cell Migration Assay -Fibronectin, 24-well, colorimetric/ECM580/1 kitSufficient for an

Millipore/ECM580 | QCM Haptotaxis Cell Migration Assay -Fibronectin, 24-well, colorimetric/ECM580/1 kitSufficient for an

價(jià)格: ￥5724.00 市場(chǎng)價(jià): 9540.00

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貨號(hào): ECM580

品牌: Millipore

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Description
CatalogueNumber	ECM580
Replaces	ECM500
BrandFamily	Chemicon®
TradeName	QCM Chemicon
Description	QCMHaptotaxisCellMigrationAssay-Fibronectin,24-well,colorimetric
Overview	Alsoavailable:CellComb?ScratchAssay!Getbiochemicaldatafromascratchassay!ClickHere Introduction TheCHEMICONQCM?HaptotaxisCellMigrationAssay-Fibronectin,Colorimetricisidealforthestudyofepithelialandfibroblastcellhaptotaxis.Thequantitativenatureofthisassayisespeciallyusefulforlargescalescreeningofpharmacologicagents.BSA-coatedcontrolchambersprovideanappropriatemigrationcontrol.The8μmporesizeofthisassay"sBoydenchambersisnotappropriateforthestudyoflymphocytemigration. ?Eachkitprovidessufficientmaterialsfortheevaluationof12samples. ?Pleaserefertotheproductinsertforassaydetails. TheCHEMICONQCM?HaptotaxisCellMigrationAssay-Fibronectin,Colorimetricassayisintendedforresearchuseonly;notfordiagnosticapplications.
MaterialsRequiredbutNotDelivered	1.PrecisionPipettes:sufficientforaliquotingcells. 2.Harvestingbuffer:EDTAortrypsinbasedcelldetachmentbuffer,orothercelldetachmentformulationsasoptimizedbyindividualinvestigators.Chemicon"sready-to-usenon-mammaliandetachmentsolution,Accutase?(Cat.No.SCR005)canalsobeused. 3.Tissueculturegrowthmediumappropriateforsubjectcells,suchasDMEMcontaining5%FBS. 4.QuenchingMedium:serum-freemedium,suchasDMEM,MEMetccontaining5%BSA.Mustcontaindivalentcations(Mg²⁺,Ca²⁺)sufficientforquenchingEDTAinharvestingbuffer. 5.SterilePBSorHBSStowashcells. 6.Distilledwater 7.(Optional)Chemoattractantorpharmacologicalagentaddedtoculturemedium. 8.Lowspeedcentrifugeandtubesforcellharvesting. 9.CO₂incubatorappropriateforsubjectcells. 10.Hemocytometerorothermeansofcountingcells. 11.TrypanblueorequivalentviABIlitystain. 12.Microplatereader(540-570nmdetection)orspectrophotometer. 13.Sterilecellculturehood 14.(Optional)Graduatedocular(calibrated),orautomatedmethodforcountingstainedcellsonamembrane. 15.Shaker

ProductInformation
Components	FibronectinTestPlate:(PartNo.2005689)One24-wellcultureplate,containing12humanFN-coatedBoydenchambers,sufficientfortheevaluationof12testsamples. BSAControlPlate:(PartNo.2005791)One24-wellcultureplate,containing12BSA-coatedBoydenchambers,sufficientfortheevaluationof12controls. CellStainSolution:(PartNo.20294)Onevial-10mL. ExtractionBuffer:(PartNo.20295)Onevial-10mL. 24wellStainExtractionPlate:(PartNo.2005871).Oneplate. 96wellStainQuantitationPlate:(PartNo.2005870).Oneplate. Swabs:(PartNo.10202)50each. Forceps:(PartNo.10203)1pair CellStainSolutioncontainsasmallamountofcrystalviolet,whichistoxicifswallowedorinhaled,andmaycauseirritationtotheeyes,respiratorysystem,andskin.Handlewithcaution.
Detectionmethod	Chromogenic

ProductInformation

Components

FibronectinTestPlate:(PartNo.2005689)One24-wellcultureplate,containing12humanFN-coatedBoydenchambers,sufficientfortheevaluationof12testsamples.
BSAControlPlate:(PartNo.2005791)One24-wellcultureplate,containing12BSA-coatedBoydenchambers,sufficientfortheevaluationof12controls.
CellStainSolution*:(PartNo.20294)Onevial-10mL.
ExtractionBuffer:(PartNo.20295)Onevial-10mL.
24wellStainExtractionPlate:(PartNo.2005871).Oneplate.
96wellStainQuantitationPlate:(PartNo.2005870).Oneplate.
Swabs:(PartNo.10202)50each.
Forceps:(PartNo.10203)1pair
*CellStainSolutioncontainsasmallamountofcrystalviolet,whichistoxicifswallowedorinhaled,andmaycauseirritationtotheeyes,respiratorysystem,andskin.Handlewithcaution.

Detectionmethod

Chromogenic

StorageandShippingInformation
StorageConditions	Storeat4°Cforuptooneyearafterdateofpurchase.

Applications
Application	TheQCMHaptotaxisCellMigrationAssay,24-wellplate-Fibronectin,Colorimetricisidealforthestudyofepithelial&fibroblastcellhaptotaxis.

BIOLOGicalInformation
SpeciesReactivity	Vertebrates

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

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