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當(dāng)前位置： Millipore >>> Millipore/DET-HP1000 | IHC Select? HRP Detection Set, (Blocking Reagent, Biotinylated linking Riagent and SA_HRP), Predi

Millipore/DET-HP1000 | IHC Select? HRP Detection Set, (Blocking Reagent, Biotinylated linking Riagent and SA_HRP), Predi

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貨號(hào): DET-HP1000

品牌: Millipore

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Description
CatalogueNumber	DET-HP1000
Replaces	2240
BrandFamily	Chemicon®
TradeName	IHCSelect Chemicon
Description	IHCSelect?HRPDetectionSet,(BlockingReagent,BiotinylatedlinkingRiagentandSA_HRP),Prediluted
Overview	IntendedUse TheIHCSelect?ImmunoperoxidaseSecondaryDetectionSystemisintendedforusewiththeCHEMICONIHCSelect?PredilutedPrimaryAntibodyreagents,whichcontainrabbitandmouseIgGforthequalitativeidentificationofantigensbylightmicroscopyinparaffin-embeddedtissues.TheIHCSelect?ImmunoperoxidaseSecondaryDetectionSystemmayalsobeusedwithotherrabbitormouseprimaryantibodies. GeneralPurposeReagents PrincipleofProcedure: TheIHCSelect?ImmunoperoxidaseSecondaryDetectionSystemusesbiotin-avidin-HRPcomplexedantibodiestodetectrabbitormouseIgGinthePrimaryAntibody.Theprimaryantibodybindstospecificantigenspresentinthespecimen.Anyexcessantibodyisremovedbywashing.Next,theSecondaryAntibodiesReagent(conjugatedtobiotin)isaddedandwillreactwiththeprimaryantibodypresentonthetissue.Unboundantibodyisremovedbywashing.ThesampleisthenbyincubatedwiththeStreptavidin-HRPsolution,whichbindstothebiotin-labeledsecondaryantibodypresentonthetissue.Unboundenzymeisremovedbywashing.Thechromogenicdevelopmentreagent,3,3"diaminobenzidine(DABSubstrate),isthenaddedandreactswiththeHRPattachedtotheHRP-streptavidin-biotin-antibodycomplex.TheHRPactivityonthechromogenicsubstrateresultsinthedepositofbrowntoblackinsolubleprecipitateatthoseantigenicsitescontainingthespecificepitopesrecognizedbytheprimaryantibody.Tissuesprocessedinavarietyoffixativesincludingneutralbufferedformalin,B-5,andBouin"smaybeused. SummaryandExplanation: Immunohistochemicaltechniqueshavebeeninusefortherecognitionofcellsintissuesectionsforatleast50years.Thefirstmethodreporteduseoffluorescentlabelsin19411.In1974,reportsofusingenzymaticlabels,suchashorserADIshperoxidase(HRP)inroutineH&Eparaffinsectionsappeared2,3.Themethodshavesincebecomethe"standardofcare"insurgicalpathologywhenclassicmethodsalonefailtoyieldadefinitivediagnosis4-9. Earlytechniqueswerebasedontheperoxidase-anti-peroxidase(PAP)reaction,whileimprovementsexploitedthestrongaffinityofavidinforbiotin10.ThistechniqueusesHRP-labeledstreptavidin.ThesecondaryantibodyisconjugatedwithbiotinandtheStrepavidin-HRPcomplexreactswiththebiotinonthesecondaryantibodies.Theresultingbiotin-avidin-HRPcomplexcanreactwiththeprimaryantibodyboundtothespecificepitopeofthetargetantigen.TheHRPenzymesofthestreptavidincomplexthencatalyzethesubstrate/chromogenreactiontoformacolouredreactionproduct(browntoblackwhenusingDABasthechromogen)attheantigensite.ABIOLOGicalstain(counterstain)isthenusedtovisualizethetissuestructure.
MaterialsRequiredbutNotDelivered	·PrimaryrabbitormouseIgGantibodies(suchasCHEMICONIHCSelect?PredilutedPrimaryAntibodies) ·WashBuffer ·Chromogen ·Counterstain ·EndogenousBlockingAgent ·Positiveandnegativetissuecontrolslides ·Positivelychargedmicroscopeslides ·Glasscoverslips(24x40mm,No.1) ·Distilledordeionizedwater ·Permanentmountingmedium,Eukitt*AmericanHistologyorequivalent ·3%laboratorygradehydrogenperoxide ·10.0%neutralbufferedformalin15 ·Xylene,ACSgrade ·Ethanol,absolute(100%) ·Latex,rubberor100%nitrilegloves(xyleneresistant) ·Timer ·Fumehood ·LightmicroscopeequippedwithPlanAchromatorequivalentlenses ·60°Cdryingoven

ProductInformation
Detectionmethod	Chromogenic

StorageandShippingInformation
StorageConditions	Whenstoredat2-8°C,theIHCSelect?ImmunoperoxidaseSecondaryDetectionSystemisstableuptotheexpirationdateprintedonthekitlabel.Donotfreezeorexposetoelevatedtemperatures.Discardanyremainingreagentsaftertheexpirationdate.Donotstorekitcomponentsorperformstaininginstronglight,suchasdirectsunlight. Precautions: ·Pooling,substitutionoralterationofanyreagentmaycauseerroneousresults. Donotmixreagentsfromdifferentlots. Donotallowtheslidestodryatanytimeduringthestainingprocedure. Handleallspecimensandmaterialscomingincontactwiththemaspotentiallyinfectiousmaterials.Disinfectwith0.05%sodiumhypochlorite. DonotmouthPipettereagents.Ifingested,seekprofessionalassistanceorcontactaPoisonControlCentreimmediately. ·Diaminobenzidine(DAB)maybecarcinogenic.Thissolutionmaycauseirritationuponskincontact.Avoidinhalation,ingestion,orskincontactandweargloveswhenhandlingDAB.Ifskincontactoccurs,flushaffectedareawithwater.Spillsmaybe"neutralized"withbleach25,26.Disposeofaccordingtolocalregulations. ·Inhalationoringestionofxyleneorthehighlyallergenicfixativeformaldehydeisharmful.Thesematerialsshouldbehandledwithgloves.Prepareinafumehood.Ifskinoreyecontactoccurs,washthoroughlywithcopiousamountsofwater. ·Weardisposablegloveswhenhandlinganyhumanmaterialandwhileperformingthestainingprocedure.

StorageandShippingInformation

StorageConditions

Whenstoredat2-8°C,theIHCSelect?ImmunoperoxidaseSecondaryDetectionSystemisstableuptotheexpirationdateprintedonthekitlabel.Donotfreezeorexposetoelevatedtemperatures.Discardanyremainingreagentsaftertheexpirationdate.Donotstorekitcomponentsorperformstaininginstronglight,suchasdirectsunlight.

Precautions:

·Pooling,substitutionoralterationofanyreagentmaycauseerroneousresults.

*Donotmixreagentsfromdifferentlots.

*Donotallowtheslidestodryatanytimeduringthestainingprocedure.

*Handleallspecimensandmaterialscomingincontactwiththemaspotentiallyinfectiousmaterials.Disinfectwith0.05%sodiumhypochlorite.

*DonotmouthPipettereagents.Ifingested,seekprofessionalassistanceorcontactaPoisonControlCentreimmediately.

·Diaminobenzidine(DAB)maybecarcinogenic.Thissolutionmaycauseirritationuponskincontact.Avoidinhalation,ingestion,orskincontactandweargloveswhenhandlingDAB.Ifskincontactoccurs,flushaffectedareawithwater.Spillsmaybe"neutralized"withbleach25,26.Disposeofaccordingtolocalregulations.

·Inhalationoringestionofxyleneorthehighlyallergenicfixativeformaldehydeisharmful.Thesematerialsshouldbehandledwithgloves.Prepareinafumehood.Ifskinoreyecontactoccurs,washthoroughlywithcopiousamountsofwater.

·Weardisposablegloveswhenhandlinganyhumanmaterialandwhileperformingthestainingprocedure.

Applications
Application	IHCSelectHRPDetectionSet,(BlockingReagent,BiotinylatedlinkingRiagent&SA_HRP),PredilutedforImmunohistochemistry(Paraffin).
KeyApplications	Immunohistochemistry(Paraffin)

BiologicalInformation
SpeciesReactivity	Mouse Rabbit

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

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