Cyanine5hydrazideisareactivedyeforthelabelingofaldehydesandketones,ananalogofCy5®hydrazide.
Thisdyereactssmoothlyandnearlyquantitativelywithvariouscarbonylgroupsencounteredinbiomolecules.Examplesareproteinssubjectedtooxidativestress,glycosylatedproteinspre-activatedbyperiodateoxidation(includingantobodies),andoligonucleotideswithaldehydemoieties.
Cyanine5hydrazidereplacescarbonyl-reactiveCy5®,AlexaFluor647,DyLight649dyes.
Generalproperties
| Appearance: | darkbluepowder | 
| Molecularweight: | 569.61 | 
| Molecularformula: | C32H42Cl2N4O | 
| Solubility: | moderatesolubilityinwater,goodinpolarorganicsolvents(DMF,DMSO,alcohols) | 
| Qualitycontrol: | NMR1H,HPLC-MS(95%) | 
| Storageconditions: | Storage:24monthsafterreceivalat-20°Cinthedark.Transportation:atroomtemperatureforupto3weeks.Avoidprolongedexposuretolight.Desiccate. | 
Spectralproperties
| Excitationmaximum,nm: | 646 | 
| ε,L⋅mol−1⋅cm−1: | 250000 | 
| Emissionmaximum,nm: | 662 | 
| Fluorescencequantumyield: | 0.2 | 
| CF260: | 0.03 | 
| CF280: | 0.04 |