ThiolreactiveBDPFLmaleimideisareactivedyeforproteinlabeling,whichhasidenticalstructurewithBODIPY®FLmaleimide.
BDPFLisaborondipyrromethenedyewhichhasabsorptionandfluorescencespectrasimilartofluorescein(FAM).However,thisdyeexhibitsveryhighphotostABIlity.Itisnon-charged,andhaslowmolecularweight.Itsbrightnessissimilartofluorescein,R110andxanthenedyederivativeslikeAlexaFluor®488.
Thisfluorophoreisidealforfluorescentmicroscopyandmanyotherapplications.Thefluorophorecansubstitutefluoresceinforalmostanyapplication,anditiscompatIBLewithanyFAM-capablefluorescentinstrumentation.
Generalproperties
| Appearance: | orangesolid | 
| MassspecM+increment: | 414.1 | 
| Molecularweight: | 414.21 | 
| Molecularformula: | C20H21BF2N4O3 | 
| Solubility: | Goodinorganicsolvents(DMF,DMSO),limitedinwater | 
| Qualitycontrol: | NMR1H,HPLC-MS(95%) | 
| Storageconditions: | Storage:24monthsafterreceivalat-20°Cinthedark.Transportation:atroomtemperatureforupto3weeks.Avoidprolongedexposuretolight.Desiccate. | 
Spectralproperties
| Excitationmaximum,nm: | 503 | 
| ε,L⋅mol−1⋅cm−1: | 80000 | 
| Emissionmaximum,nm: | 509 | 
| Fluorescencequantumyield: | 0.97 | 
| CF260: | 0.015 | 
| CF280: | 0.027 |