Watersoluble,amino-reactivesulfo-Cyanine3NHSester.Efficientlylabelsproteinsandpeptidesinpurelyaqueoussolution,withoutneedfororganicco-solvent.Idealforproteinswithlowsolubility,andproteinspronetodenaturation.
Thisissulfonated,hydrophilicandwater-solubledye.Non-sulfonatedCyanine3NHSesterisalsoavailable.
ThisproductisananalogofCy3®NHSester.Sulfo-Cyanine3NHSesterreplacesCy3®,AlexaFluor546,andDyLight549forallapplications.
Generalproperties
| Appearance: | darkredcrystals | 
| Molecularweight: | 735.80 | 
| Molecularformula: | C34H38N3NaO10S2 | 
| Solubility: | veryhighinwater,goodinpolarorganicsolvents(DMF,DMSO) | 
| Qualitycontrol: | NMR1H,HPLC-MS(95%) | 
| Storageconditions: | Storage:12monthsafterreceivalat-20°Cinthedark.Transportation:atroomtemperatureforupto3weeks.Avoidprolongedexposuretolight.Desiccate. | 
Spectralproperties
| Excitationmaximum,nm: | 548 | 
| ε,L⋅mol−1⋅cm−1: | 162000 | 
| Emissionmaximum,nm: | 563 | 
| Fluorescencequantumyield: | 0.1 | 
| CF260: | 0.03 | 
| CF280: | 0.06 |