Cyanine3NHSesterisareactivedyeforthelabelingofamino-groupsinbiomolecules,ananalogofCy3®NHSester.Thisreagentisidealforthelabelingofsolubleproteins,peptides,andoligonucleotides/DNA.Fordelicateproteinsconsiderusingwater-solublesulfo-Cyanine3NHSesterwhichdoesnotrequireuseofanyco-solvent.
Cyanine3NHSesterisareplacementforNHSestersofCy3®,AlexaFluor546,andDyLight549.
Generalproperties
| Appearance: | redpowder | 
| MassspecM+increment: | 474.2 | 
| Molecularweight: | 641.5 | 
| CASnumber: | 1032678-38-8,1032815-92-1 | 
| Molecularformula: | C34H40N3BF4O4 | 
| Solubility: | solubleinorganicsolvents(DMF,DMSO,dichloromethane),insolubleinwater | 
| Qualitycontrol: | NMR1HandHPLC-MS(95+%) | 
| Storageconditions: | Storage:12monthsafterreceivalat-20°Cinthedark.Transportation:atroomtemperatureforupto3weeks.Avoidprolongedexposuretolight.Desiccate. | 
Spectralproperties
| Excitationmaximum,nm: | 555 | 
| ε,L⋅mol−1⋅cm−1: | 150000 | 
| Emissionmaximum,nm: | 570 | 
| Fluorescencequantumyield: | 0.31 | 
| CF260: | 0.04 | 
| CF280: | 0.09 |