Thiol-reactive,nearinfrareddyeCyanine7.5forthelabelingofproteinSHgroups,ananalogofCy7.5®maleimide.
Mostproteinscontainsulfhydrylgroupswhichcanbeselectivelylabeledwithmaleimides.Withthisreagent,theseproteinscanbeconvertedtonearinfrared(NIR)fluorescentconjugates.Afterit,theconjugatescanbeusedinnon-invasiveliveorganismimagingtostudydistributionoftheproteinintissuesandorgans.
Generalproperties
| Appearance: | greenpowder | 
| Molecularweight: | 807.46 | 
| Molecularformula: | C51H55ClN4O3 | 
| Solubility: | solubleinorganicsolvents(DMSO,DMF,dichloromethane),lowsolubilityinwater | 
| Qualitycontrol: | NMR1H,HPLC-MS(95%) | 
| Storageconditions: | Storage:24monthsafterreceivalat-20°Cinthedark.Transportation:atroomtemperatureforupto3weeks.Avoidprolongedexposuretolight.Desiccate. | 
Spectralproperties
| Excitationmaximum,nm: | 788 | 
| ε,L⋅mol−1⋅cm−1: | 223000 | 
| Emissionmaximum,nm: | 808 |