Fluorescein(FAM)isabrightfluorophorethathasfoundanextremelywideapplicationinlifescienceresearch.Thefluorophoreishydrophilicanditsderivativespossessgoodsolubilityinaqueousbuffers.
FluoresceiniscompatIBLewithawidevarietyoffluorescentinstrumentation-mostinstrumentsareequippedwithFAMchannelfilters.
Thisproductcontainsthepure6-isomeroffluorescein.
Recommendedprotocol
NHSesterlabelingofaminobiomolecules
Generalproperties
| Appearance: | yellowcrystallinesolid | 
| MassspecM+increment: | 358.0 | 
| Molecularweight: | 473.39 | 
| Molecularformula: | C25NO9H15 | 
| IUPACname: | (2,5-dioxopyrrolidin-1-yl)3",6"-dihydroxy-1-oxospiro[2-benzofuran-3,9"-xanthene]-5-carboxylate | 
| Solubility: | goodinDMF,DMSO | 
| Qualitycontrol: | NMR1H,HPLC-MS(95%) | 
| Storageconditions: | Storage:12monthsafterreceivalat-20°Cinthedark.Transportation:atroomtemperatureforupto3weeks.Avoidprolongedexposuretolight.Desiccate. | 
Spectralproperties
| Excitationmaximum,nm: | 494 | 
| ε,L⋅mol−1⋅cm−1: | 75000 | 
| Emissionmaximum,nm: | 520 | 
| Fluorescencequantumyield: | 0.9 | 
| CF260: | 0.20 | 
| CF280: | 0.17 |