| Overview | TheGuavaCaspaseAssaySuiteoffluorescent-basedassaysforCaspase 3/7,8,9andmulti-caspaseemploythesameprotocolmakingiteasier
 toperformmultiplecaspaseassaysinparallel.Further,Guavacaspase
 reagentsaremembranepermeableallowingonetomeasureintracellular
 levelsoftheenzymewithoutusingharshlysismethods.TheGuava
 CaspaseAssaysemployaFLICA(FluorescentLabeledInhibitorof
 Caspase)reagentthatspecificallyidentifiesactivecaspases.EachFLICAis
 comprisedofthreesubunits:
 –anactivesitebindingpeptidetoconferspecificity(DEVDforcaspase3/7,
 LETDforcaspase8,LEHDforcaspase9,andVADformulti-caspase)
 –acovalentcrosslinkingagent(FMK)
 –agreen(FAM)ororange-redfluorescentprobe(SR):SRisexcitedbyblue
 orgreenlasers(PCASystemsorEasyCyteSystems),FAMbybluelasers
 only(EasyCyteSystems)
 TheGuavaCaspaseAssaysalsoincludethenuclearDNAstain,7-AAD,to
 simultaneouslyevaluatemembraneintegrityandcellviABIlity.
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