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當(dāng)前位置： Millipore >>> Millipore/17-474 | STAR phospho-MEK1 (Ser218/222) ELISA Kit/17-474/96 assays

Millipore/17-474 | STAR phospho-MEK1 (Ser218/222) ELISA Kit/17-474/96 assays

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貨號(hào): 17-474

品牌: Millipore

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Description
CatalogueNumber	17-474
BrandFamily	Upstate
TradeName	STAR Upstate
Description	STARphospho-MEK1(Ser218/222)ELISAKit
BackgroundInformation	TheUPSTATEcolorimetricSTAR(SignalTransductionAssayReaction)ELISAkitisasolidphasesandwichenzymelinkedimmunosorbentassaythatprovidesafast,sensitivemethodtodetectspecificlevelsofsignalingtargetsindenaturedcellextracts.TheMEK1plateiscoatedwithaspecificmousemonoclonalanti-MEK1captureantibodyonthemicrowellsofthe96-wellclearplate.SamplelysateorthestandardincludedinthekitareincubatedinthemicrowellsallowingMEK1antigentobecapturedintheplatewells.Theplateisthenwashedtoremoveanynon-boundunspecificmaterial.Thewellsarethenincubatedwithaspecificrabbitanti-phospho-MEK1(Ser218/222)antibodytodetectthecapturedontheplatewell.TheunbounddetectionantibodyiswashedawayfollowedbyincubationwithanHRP-conjugatedanti-rabbitantibody.Thisallowsforasensitiveenzymaticdetectionofthesample.AftertheadditionofTMBsubstrateandstopsolutiontheabsorbanceismeasuredat450nmusingaplatereader.Theentireassaytakeslessthan5hourstocompletewithminimalhands-ontime.Manyofthereagentsaresuppliedinready-touseformulationsforeaseofuse.Thekitalsoincludesastandardthatisrunasbothapositivecontrolandtodevelopastandardcurve.
MaterialsRequiredbutNotDelivered	1.Multi-channelorrepeatingPipettes 2.Plateshaker(optional) 3.Pipettors&tipscapableofaccuratelymeasuring1-1000%micro;L 4.GraduatedSEROlogicalpipettes 5.96-wellmicrotiterPlateReaderwith450nmfilter 6.Graphingsoftwareforplottingdataorgraphpaperformanualplottingofdata 7.Microfugetubesforstandardandsampledilutions 8.Mechanicalvortex 9.1litercontainer 10.Distilledordeionizedwater

ProductInformation
Components	1.CapturePlatepre-coatedwithanti-MEK1antibody:(PartNo.17-474A)Onepre-coated96-stripwellimmunoplatesealedinafoilpouch. 2.Anti-phospho-MEK1(Ser218/222)detectionantibody:(PartNo.17-474B)Onebottle(11mL)ofanti-phospho-MEK1(Ser218/222)detectionantibodycontainingsodiumazide,readytouse. 3.ELISADiluent:(PartNo.17-474C)Onebottle(25mL)ofELISADiluentcontainingsodiumazide,readytouse. 4.25XELISAWashBuffer:(PartNo.17-474D)Onebottle(50mL)of25XELISAWashBuffer. 5.Anti-RabbitIgGHRPconjugate:(PartNo.17-474E)Onevial(125μL)of100Xanti-rabbitHRPconjugatecontainingthimerosol. 6.HRPDiluent:(PartNo.17-474F)Onebottle(25mL)ofHRPDiluentcontainingthimerosol. 7.TMBSolution:(PartNo.17-474G)Onebottle(25mL)ofstABIlizedtetramethylbenzidine(TMB),readytouse. 8.StopSolution:(PartNo.17-474H)Onebottle(25mL)ofstopsolution,readytouse. 9.Phospho-MEK1(Ser218/222Standard:(PartNo.17-474I)Twovialsofphospho-MEK1(Ser218/222)standard,lyophilized. 10.SampleIncubationBuffer:(PartNo.17-73J)Onebottle(10mL)ofasampleincubation(denaturing)buffer,readytouse. 11.PlateCovers:Twoplatecovers.
Detectionmethod	Chromogenic

ProductInformation

Components

1.CapturePlatepre-coatedwithanti-MEK1antibody:(PartNo.17-474A)Onepre-coated96-stripwellimmunoplatesealedinafoilpouch.
2.Anti-phospho-MEK1(Ser218/222)detectionantibody:(PartNo.17-474B)Onebottle(11mL)ofanti-phospho-MEK1(Ser218/222)detectionantibodycontainingsodiumazide,readytouse.
3.ELISADiluent:(PartNo.17-474C)Onebottle(25mL)ofELISADiluentcontainingsodiumazide,readytouse.
4.25XELISAWashBuffer:(PartNo.17-474D)Onebottle(50mL)of25XELISAWashBuffer.
5.Anti-RabbitIgGHRPconjugate:(PartNo.17-474E)Onevial(125μL)of100Xanti-rabbitHRPconjugatecontainingthimerosol.
6.HRPDiluent:(PartNo.17-474F)Onebottle(25mL)ofHRPDiluentcontainingthimerosol.
7.TMBSolution:(PartNo.17-474G)Onebottle(25mL)ofstABIlizedtetramethylbenzidine(TMB),readytouse.
8.StopSolution:(PartNo.17-474H)Onebottle(25mL)ofstopsolution,readytouse.
9.Phospho-MEK1(Ser218/222Standard:(PartNo.17-474I)Twovialsofphospho-MEK1(Ser218/222)standard,lyophilized.
10.SampleIncubationBuffer:(PartNo.17-73J)Onebottle(10mL)ofasampleincubation(denaturing)buffer,readytouse.
11.PlateCovers:Twoplatecovers.

Detectionmethod

Chromogenic

StorageandShippingInformation
StorageConditions	2yearsat4°C

Applications
KeyApplications	ELISA

BIOLOGicalInformation
SpeciesReactivity	Human Mouse
AnalytesAvailable	MEK1
EntrezGeneNumber	NM_002755.2
EntrezGeneSummary	Theproteinencodedbythisgeneisamemberofthedualspecificityproteinkinasefamily,whichactsasamitogen-activatedprotein(MAP)kinasekinase.MAPkinases,alsoknownasextracellularsignal-regulatedkinases(ERKs),actasanintegrationpointformultiplebiochemicalsignals.ThisproteinkinaseliesupstreamofMAPkinasesandstimulatestheenzymaticactivityofMAPkinasesuponwidevarietyofextra-andintracellularsignals.AsanessentialcomponentofMAPkinasesignaltransductionpathway,thiskinaseisinvolvedinmanycellularprocessessuchasproliferation,differentiation,transcriptionregulationanddevelopment.
GeneSymbol	MAP2K1 MAPKK1 PRKMK1 MEK1 MKK1
Modifications	Phosphorylation
UniProtNumber	Q02750
UniProtSummary	FUNCTION:SwissProt:Q02750#CatalyzestheconcomitantphosphorylationofathreonineandatyrosineresidueinaThr-Glu-TyrsequencelocatedinMAPkinases.ActivatesERK1andERK2MAPkinases. SIZE:393aminoacids;43439Da SUBUNIT:InteractswithMORG1(Bysimilarity).InteractswithYersiniayopJ. PTM:PhosphorylationonSer/ThrbyMAPkinasekinasekinases(RAForMEKK1)regulatespositivelythekinaseactivity.&AcetylationbyYersiniayopJpreventsphosphorylationandactivation,thusblockingtheMAPKsignalingpathway. DISEASE:SwissProt:Q02750#DefectsinMAP2K1areacauseofcardiofaciocutaneoussyndrome(CFCsyndrome)[MIM:115150];alsoknownascardio-facio-cutaneoussyndrome.CFCsyndromeischaracterizedbyadistinctivefacialappearance,heartdefectsandmentalretardation.Heartdefectsincludepulmonicstenosis,atrialseptaldefectsandhypertrophiccardiomyopathy.Someaffectedindividualspresentwithectodermalabnormalitiessuchassparse,friablehair,hyperkeratoticskinlesionsandageneralizedichthyosis-likecondition.TypicalfacialfeaturesaresimilartoNoonansyndrome.Theyincludehighforeheadwithbitemporalconstriction,hypoplasticsupraorbitalridges,downslantingpalpebralfissures,adepressednasalbridge,andposteriorlyangulatedearswithprominenthelices.TheinheritanceofCFCsyndromeisautosomaldominant. SIMILARITY:SwissProt:Q02750##Belongstotheproteinkinasesuperfamily.STESer/Thrproteinkinasefamily.MAPkinasekinasesubfamily.&Contains1proteinkinasedomain.

PhysicochemicalInformation
Sensitivity	Sensitivity:0.75Units/mL. RangeofDetection:1.6to100Units/mL

Dimensions

MaterialsInformation

MaterialsInformation

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