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當(dāng)前位置： Millipore >>> Millipore/17-10054 | ChIPAb+ Histone H2B - ChIP Validated Antibody and Primer Set/17-10054/25 assays25 assays per set. R

Millipore/17-10054 | ChIPAb+ Histone H2B - ChIP Validated Antibody and Primer Set/17-10054/25 assays25 assays per set. R

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Description
CatalogueNumber	17-10054
BrandFamily	Upstate
TradeName	ChIPAb+ Upstate
Description	ChIPAb+HistoneH2B-ChIPValidatedAntibodyandPrimerSet
Overview	AllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction. TheChIPAb+HistoneH2BsetincludestheHistoneH2Bantibody,anegativecontrolrabbitIgG,andqPCRprimerswhichamplifya110bpregionofhumanβ-globinpromoter.TheHistoneH2Bandnegativecontrolsaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationofHistoneH2B-associatedchromatin.
AlternateNames	H2B HistoneH2B H2Bhistonefamily,memberQ histone2,H2be histonecluster2,H2be
BackgroundInformation	HistoneH2Bisoneofthe5mainhistoneproteinsinvolvedinthestructureofchromatinineukaryoticcells.FeaturingamainglobulardomainandalongNterminaltailH2Aisinvolvedwiththestructureofthenucleosomesofthe"beadsonastring"structure.

ProductInformation
Format	Purified
Control	IncludesnegativecontrolnormalrabbitIgGantibodyandprimersspecificforhumanβ-globin.
Presentation	Anti-HistoneH2B(rabbitpolyclonal).Onevialcontaining25μLofproteinApurifiedrabbitpolyclonalinbuffercontaining0.02Mphosphatebuffer,pH7.6,0.25MNaCl,and0.1%sodiumazide,and30%glycerol.Storeat-20°C. NormalRabbitIgG.Onevialcontaining125μgofpurifiedrabbitIgGin125μLstoragebuffer.Storeat-20°C. ChIPPrimers,β-globin.Onevialcontaining75μLof5μMofeachprimerspecificforthehumanβ-globinpromoter.Storeat-20°C. FOR:AGGACAGGTACGGCTGTCATC REV:TTTATGCCCAGCCCTGGCTC

StorageandShippingInformation
StorageConditions	Stablefor1yearat-20°Cfromdateofreceipt.HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIllityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.

Applications
Application	TheChIPAb+HistoneH2BsetincludestheHistoneH2Bantibody,anegativecontrolrabbitIgG&qPCRprimerswhichamplifya110bpregionofhumanβ-globinpromoter.
KeyApplications	WesternBlotting ChromatinImmunoprecipitation(ChIP)
ApplicationNotes	ChromatinImmunoprecipitation: SonicatedchromatinpreparedfromHeLacells(1X10⁶cellequivalentsperIP)weresubjectedtochromatinimmunoprecipitationusingeither1μLofNormalRabbitIgGor1μLofAnti-HistoneH2BandtheMagnaChIP?AKit(Cat.#17-610). SuccessfulimmunoprecipitationofHistoneH2BassociatedDNAfragmentswasverifiedbyqPCRusingChIPPrimers,humanβ-globinforapositive,andhumanGAPDHprimersasanegativeassay(Pleaseseefigures). DataispresentedaspercentinputofeachIPsamplerelativetoinputchromatinforeachampliconandChIPsampleasindicated. PleaserefertotheEZ-MagnaChIP?A(Cat.#17-408)orEZ-ChIP?(Cat.#17-371)protocolforexperimentaldetails. WesternBlotAnalysis: Representativelotdata. RecombinantXenopusHistoneH2B,chickencorehistonesandacidextractfromeithersodiumbutyrateorcolcemidtreatedHeLacellswereresolvedbyelectrophoresis,transferredtonitrocelluloseandprobedwithanti-HistoneH2B(1:5,000dilution). Proteinswerevisualizedusingagoatanti-rabbitsecondaryantibodyconjugatedtoHRPandachemiluminescencedetectionsystem(Pleaseseefigures).

BiologicalInformation
Immunogen	KLH-conjugated,syntheticpeptidecorrespondingtoaminoacids118-126(CG-AVTKYTSSK)ofhumanHistoneH2B,withanN-terminalCGaddedforconjugationpurposes.
Host	Rabbit
Specificity	RecognizesandisspecificforHistoneH2B,MW~15kDa.Anadditionalbandat~23kDaisdetectedinsomesamples,whichlikelycorrespondstoubiquityl-HistoneH2B.
SpeciesReactivity	Chicken Xenopus Human Yeast(S.cerevisiae)
SpeciesReactivityNote	Human,chicken,yeastandXenopus.Othersnottested.Broadspeciescross-reactivityexpectedbasedonsequencehomology.
AntibodyType	PolyclonalAntibody
EntrezGeneNumber	NP_778225
EntrezGeneSummary	HistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Nucleosomesconsistofapproximately146bpofDNAwrappedaroundahistoneoctamercomposedofpairsofeachofthefourcorehistones(H2A,H2B,H3,andH4).Thechromatinfiberisfurthercompactedthroughtheinteractionofalinkerhistone,H1,withtheDNAbetweenthenucleosomestoformhigherorderchromatinstructures.ThisgeneisintronlessandencodesamemberofthehistoneH2Bfamily.Transcriptsfromthisgenecontainapalindromicterminationelement.
GeneSymbol	GL105 H2B H2B-GL105 H2B.1 H2B.q H2B/q H2BFQ MGC119802 MGC119804 MGC129733 MGC129734 OTTHUMP00000013920
PurificationMethod	ProteinAPurfied
UniProtNumber	Q8N257
UniProtSummary	FUNCTION:SwissProt:Q8N257#Corecomponentofnucleosome.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstability.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling. SIZE:126aminoacids;13908Da SUBUNIT:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA. SUBCELLULARLOCATION:Nucleus. PTM:MonoubiquitinationofLys-121bytheRNF20/40complexgivesaspecifictagforepigenetictranscriptionalactivationandisalsoprerequisiteforhistoneH3"Lys-4"and"Lys-79"methylation.ItalsofunctionscooperativelywiththeFACTdimertostimulateelongationbyRNApolymeraseII.&PhosphorylatedonSer-15bySTK4/MST1duringapoptosis;whichfacilitatesapoptoticchromatincondensation.AlsophosphorylatedonSer-15inresponsetoDNAdoublestrandbreaks(DSBs),andincorrelationwithsomatichypermutationandimmunoglobulinclass-switchrecombination. SIMILARITY:SwissProt:Q8N257##BelongstothehistoneH2Bfamily.
MolecularWeight	~15kDa

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