| Source | ProducedinE.coli. | 
| SpecificActivity | ForSpecificActivitydata,refertotheCertificateofAnalysisforindividuallotsofthisenzyme. | 
| EntrezGeneNumber |  | 
| EntrezGeneSummary | HistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Nucleosomesconsistofapproximately146bpofDNAwrappedaroundahistoneoctamercomposedofpairsofeachofthefourcorehistones(H2A,H2B,H3,andH4).Thechromatinfiberisfurthercompactedthroughtheinteractionofalinkerhistone,H1,withtheDNAbetweenthenucleosomestoformhigherorderchromatinstructures.ThisgeneisintronlessandencodesamemberofthehistoneH2Bfamily.Transcriptsfromthisgenecontainapalindromicterminationelement. | 
| GeneSymbol |  | 
| PurificationMethod | Sequentialchromatographyovergelfiltration,hydrophobicinteractionandionexchangeresins | 
| UniProtNumber |  | 
| UniProtSummary | FUNCTION:SwissProt:Q8N257#Corecomponentofnucleosome.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstABIlity.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling. SIZE:126aminoacids;13908Da
 SUBUNIT:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA.
 SUBCELLULARLOCATION:Nucleus.
 PTM:MonoubiquitinationofLys-121bytheRNF20/40complexgivesaspecifictagforepigenetictranscriptionalactivationandisalsoprerequisiteforhistoneH3"Lys-4"and"Lys-79"methylation.ItalsofunctionscooperativelywiththeFACTdimertostimulateelongationbyRNApolymeraseII.&PhosphorylatedonSer-15bySTK4/MST1duringapoptosis;whichfacilitatesapoptoticchromatincondensation.AlsophosphorylatedonSer-15inresponsetoDNAdoublestrandbreaks(DSBs),andincorrelationwithsomatichypermutationandimmunoglobulinclass-switchrecombination.
 SIMILARITY:SwissProt:Q8N257##BelongstothehistoneH2Bfamily.
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| MolecularWeight | 15kDa |