Millipore/06-574 | Anti-TrkA Antibody/06-574/200 µg

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貨號(hào): 06-574

品牌: Millipore

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Description
CatalogueNumber	06-574
Replaces	CBL770
BrandFamily	Upstate
TradeName	Upstate
Description	Anti-TrkAAntibody
AlternateNames	Neurotrophictyrosinekinasereceptortype OncogeneTRK TRK1-transformingtyrosinekinaseprotein highaffinitynervegrowthfactorreceptor neurotrophictyrosinekinase,receptor,type1 tyrosinekinasereceptorA
BackgroundInformation	TrkA,apparentmolecularweight140kDa,isahighaffinitynervegrowthfactor(NGF).TheTrkproto-oncogenefamilycontainsfourmembers,TrkA,TrkB,TrkC,andTrkE,whicharevariablyexpressedthroughoutthecentralandperipheralnervoussystems.TrkAbindstonervegrowthfactor(NGF)andautophosphorylatesontyrosineresidues(Tyr490,Tyr674,Tyr675,Tyr751andTyr785)toactivatemultipledownstreameffectorproteins.PhosphorylationatTyr490isrequiredforShcassociationandsubsequentactivationoftheRas-MAPkinase-signalingcascade,whichleadstoactivationofElk-1-dependentgenetranscriptionandneuritegrowth.PhosphorylationsatTyr674andTyr675liewithinthecatalyticdomainofTrkAtyrosinekinaseandreflectTrkkinaseactivity.Additionally,phosphorylationatTyr751isrequiredforPI3-kinaseassociationandactivationoftheAktsignalingcascade.

ProductInformation
Format	Purified
Control	EGF-stimulatedA431cellysate,ratbraintissue.IncludedPositiveAntigenControl:Catalog#12-301,non-stimulatedA431celllysate.Add2.5μLof2-mercaptoethanol/100μLoflysateandboilfor5minutestoreducethepreparation.Load20μgofreducedlysateperlaneforminigels.
Presentation	PurifiedrabbitIgGinbuffercontaining0.02Mphosphatebuffer,pH7.6,0.25MNaCl,0.1%sodiumazide.Frozenat-20°C.

StorageandShippingInformation
StorageConditions	Stablefor1yearat-20°Cfromdateofreceipt. HandlingRecommendations:Uponreceipt,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.

Applications
Application	Anti-TrkAAntibodydetectslevelofTrkA&hasbeenpublished&validatedforuseinIC,IP&WB.
KeyApplications	Immunocytochemistry Immunoprecipitation WesternBlotting
ApplicationNotes	Immunoprecipitation: 4μgofapreviouslotimmunoprecipitatedTrkAfrom500μgofPC-12RIPAlysate. Immunocytochemistry: 10μg/mLofapreviouslotofthisantibodyshowedpositiveimmunostainingforTrkAinPC-12cellsfixedwith4%paraformaldehyde.

BIOLOGicalInformation
Immunogen	RabbitsimmunizedwithbacteriallyexpressedpurifiedproteincorrespondingtotheentireextracellulardomainofratTrkAreceptor.
Concentration	PleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
Host	Rabbit
Specificity	RecognizesTrkA,Mr140kDa.Doesnotcross-reactwithTrkBorTrkC.
Isotype	IgG
SpeciesReactivity	Human Mouse Rat
SpeciesReactivityNote	Rat,mouseandhuman.Reactivitywithotherspecieshasnotbeenconfirmed.
AntibodyType	PolyclonalAntibody
EntrezGeneNumber	NM_021589
EntrezGeneSummary	Thisgeneencodesamemberoftheneurotrophictyrosinekinasereceptor(NTKR)family.Thiskinaseisamembrane-boundreceptorthat,uponneurotrophinbinding,phosphorylatesitselfandmembersoftheMAPKpathway.Thepresenceofthiskinaseleadstocelldifferentiationandmayplayaroleinspecifyingsensoryneuronsubtypes.Mutationsinthisgenehavebeenassociatedwithcongenitalinsensitivitytopain,anhidrosis,self-mutilatingbehavior,mentalretardationandcancer.Alternatetranscriptionalsplicevariantsofthisgenehavebeenfound,butonlythreehavebeencharacterizedtodate.
GeneSymbol	DKFZp781I14186 EC2.7.10.1 MTC OTTHUMP00000038736 TRK TRK1 TRKA Trk-A p140-TrkA
PurificationMethod	ProteinAPurfied
UniProtNumber	P04629
UniProtSummary	FUNCTION:SwissProt:P04629#Requiredforhigh-affinitybindingtonervegrowthfactor(NGF),neurotrophin-3andneurotrophin-4/5butnotbrain-derivedneurotrophicfactor(BDNF).KnownsubstratesfortheTrkreceptorsareSHC1,PI3-kinase,andPLC-gamma-1.Hasacrucialroleinthedevelopmentandfunctionofthenociceptivereceptionsystemaswellasestablishmentofthermalregulationviasweating.ActivatesERK1byeitherSHC1-orPLC-gamma-1-dependentsignalingpathway. SIZE:796aminoacids;87497Da SUBUNIT:Existsinadynamicequilibriumbetweenmonomeric(lowaffinity)anddimeric(highaffinity)structures.BindsSH2B2.InteractswithSQSTM1whichbridgesNTRK1toNGFR(Bysimilarity). SUBCELLULARLOCATION:Cellmembrane;Single-passtypeImembraneprotein(Bysimilarity).Note=EndocytosedtotheendosomesupontreatmentofcellswithNGF(Bysimilarity). TISSUESPECIFICITY:IsoformTrkA-IIisprimarilyexpressedinneuronalcells;isoformTrkA-Iisfoundinnon-neuronaltissues. PTM:Ligand-mediatedauto-phosphorylation.InteractionwithSQSTM1isphosphotyrosine-dependent. DISEASE:SwissProt:P04629#DefectsinNTRK1areacauseofcongenitalinsensitivitytopainwithanhidrosis(CIPA)[MIM:256800].CIPAischaracterizedbyacongenitalinsensitivitytopain,anhidrosis(absenceofsweating),absenceofreactiontonoxiousstimuli,self-mutilatingbehavior,andmentalretardation.ThisrareautosomalrecessivedisorderisalsoknownascongenitalsensoryneuropathywithanhidrosisorhereditarysensoryandautonomicneuropathytypeIVorfamilialdysautonomiatypeII.&ChromosomalaberrationsinvolvingNTRK1areacauseofthyroidpapillarycarcinoma(PACT)[MIM:188550].Translocationt(1;3)(q21;q11)withTFGgeneratestheTRKT3(TRK-T3)transcriptbyfusingTFGtothe3"-endofNTRK1;arearrangementwithTPM3generatestheTRKtranscriptbyfusingTPM3tothe3"-endofNTRK1.&ChromosomalaberrationsinvolvingNTRK1areacauseofthyroidpapillarycarcinoma(PACT)[MIM:188550].IntrachromosomalrearrangementthatlinkstheproteinkinasedomainofNTRK1tothe5"-endoftheTPRgeneformsthefusionproteinTRK-T1.TRK-T1isa55kDaproteinreactingwithantibodiesagainsttheC-terminusoftheNTRK1protein. SIMILARITY:SwissProt:P04629##Belongstotheproteinkinasesuperfamily.Tyrproteinkinasefamily.Insulinreceptorsubfamily.&Contains2Ig-likeC2-type(immunoglobulin-like)domains.&Contains3LRR(leucine-rich)repeats.&Contains1proteinkinasedomain....hide?seemore?
MolecularWeight	140kDa

PhysicochemicalInformation

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